Oprah Winfrey ended her final show last night with a mention of her gay fans.

The chatshow queen, who sobbed and laughed through her final hour, thanked viewers for their support over the last 25 years.

“I thank you for tuning in every day, along with your mothers and your sisters and your daughters, your partners, gay and otherwise,” she said.

“I thank you for being as much of a sweet inspiration for me as I’ve tried to be for you.”

Oprah, who is in a long-term relationship with a man, has been dogged by lesbian rumours over her relationship with best friend Gayle King.

The pair have laughed off the gossip, saying they would not keep any romance secret.

Recent LGBT guests on the show have included Ricky Martin, pregnant transman Thomas Beattie, trans supermodel Lea T and Portia di Rossi.